Instrumental Techniques - HPLC, UPLC, LCMS and GCMS

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Student Lab Practice

A 5-day short course on the instrumental techniques using HPLC, UPLC, LCMS and GCMS

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Course summary

  • Duration: 5 days
  • Experience level: Intermediate to advanced
  • Course type: Universal, applicable to all instrumentation
  • Teaching method: 100% Face-to-Face lecture/ workshop sessions in classroom and 100% practical exercises in the laboratory
  • Course venue: School of Health, Sport and Bioscience, University of East London, Stratford Campus, Water Lane, London E15 4LZ
  • Course fee: £1,000 per participant (external); 50% off for UEL students and UEL Alumni graduated from the School of Health, Sport and Bioscience

Course Description

Who is course for: Recent graduates and levels 6, 7 and 8 students who are looking for a job in pharmaceutical and Biotechnology companies, food industries, healthcare diagnostics, environment agencies and research organisations. This course will accelerate the analytical skills and boost confidence in analysing data; therefore, it would be very useful for those who have been working in industry but are willing to upskill their capabilities.

Learning objectives: By the end of this course, you will have a developing capacity to:

  1. understand the basic principles of HPLC, UPLC, LCMS and GCMS 
  2. be familiar with sample preparation for HPLC, UPLC, LCMS and GCMS analysis
  3. be familiar with how HPLC, UPLC, LCMS and GCMS works
  4. have practical experience of adjusting the parameters for each HPLC component
  5. have practical experience of trouble shooting during the operation of HPLC, UPLC, LCMS and GCMS
  6. be confident with the calculations related to sample preparation
  7. be confident on the data analysis

What will you learn on this course?

The 5 day course offers the use of our in house state-of-the-art equipment. We will train the participants on four key instruments: HPLC, UPLC, LCMS and GCMS including applications, method developments, troubleshooting and maintenance.

The course is designed to cover all aspects of practical theory that the participants would need to know about liquid and gas chromatography and related techniques including liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry.

We will offer intensive training to the participants on the sample preparation, mobile phase optimisation (both isocratic and gradient systems), pumps, method development, autosampler injection techniques, columns, pre-columns, detectors, calibration standards, data analysis for both quantitation and qualitative analysis.

Applications will include specific quantification of metabolites and endogenous compounds in plant material, pharmaceutical products and body fluids, metabolic profiling and metabolomics.